Mutation ampE729(del)::kan
- Name: ampE729(del)::kan
- Type: Insertion
- Mutation of Gene: ampE
- Approx. Map Location: 2.57
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGATTCCGGGGATCCGTCGACC and TCACCCGTACAGGGTGAGGGCAATAATCTTTACACCAACGCCCCGTAAATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0107-1 7 F-, Δ(araD-araB)567, ΔampE729::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514