Mutation argI728(del)::kan
- Name: argI728(del)::kan
- Type: Insertion
- Mutation of Gene: argI
- Approx. Map Location: 96.46
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACATTCCGGGGATCCGTCGACC and ATAAATTGAATTTTAATTCATTGAGGCGTTAGCCACAGGAGGGATCTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4211-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔargI728::kan, hsdR514