Mutation cof-732(del)::kan
- Name: cof-732(del)::kan
- Type: Insertion
- Mutation of Gene: cof
- Approx. Map Location: 10.06
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGATTCCGGGGATCCGTCGACC and GCAATTGCCCGGTGCTGGAAGGGATCTCGTTATTCGGGGGAATAAGGTAGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0436-3 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δcof-732::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514