Mutation cpdA729(del)::kan
- Name: cpdA729(del)::kan
- Type: Insertion
- Mutation of Gene: cpdA
- Approx. Map Location: 68.42
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATTCCGGGGATCCGTCGACC and CGATGGCGATTCCGGTTTATTAGCGTCGTGAAACCTAAGGACACCATTTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3000-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔcpdA729::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514