Mutation cyaA751(del)::kan
- Name: cyaA751(del)::kan
- Type: Insertion
- Mutation of Gene: cyaA
- Approx. Map Location: 85.98
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CGGATAAGCCTCGCTTTCCGGCACGTTCATCACGAAAAATATTGCTGTAAATTCCGGGGATCCGTCGACC and GTTGGCGGAATCACAGTCATGACGGGTAGCAAATCAGGCGATACGTCTTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3778-3 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔcyaA751::kan, Δ(rhaD-rhaB)568, hsdR514