Mutation dsbB774(del)::kan
- Name: dsbB774(del)::kan
- Type: Insertion
- Mutation of Gene: dsbB
- Approx. Map Location: 26.55
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGATTCCGGGGATCCGTCGACC and GAATTGGTTTAAACTGCGCACTCTATGCATATTGCAGGGAAATGATTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5182-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔdsbB774::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514