Mutation frsA757(del)::kan
- Name: frsA757(del)::kan
- Type: Insertion
- Mutation of Gene: frsA
- Approx. Map Location: 5.53
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGATTCCGGGGATCCGTCGACC and TTACCAAATTGGCAAAATTTAGCAAATTTTTAACACAAGCGTTTTTCGATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0229-2 7 F-, Δ(araD-araB)567, ΔfrsA757::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514