Mutation hisB720(del)::kan
- Name: hisB720(del)::kan
- Type: Insertion
- Mutation of Gene: hisB
- Approx. Map Location: 45.08
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGATTCCGGGGATCCGTCGACC and GCAGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2004-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔhisB720::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514