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Mutation - ileS741(del)::kanMutation ileS741(del)::kan Mutation ileS741(del)::kan
- Name: ileS741(del)::kan
- Type: Insertion
- Mutation of Gene: ileS
- Approx. Map Location: 0.48
- Properties
Property | Symbol |
---|
deletion | del |
kanamycin resistant | |
- Comments
- The primers used to make this knockout were CTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGATTCCGGGGATCCGTCGACC and TAGCCCTGTTGAACAGATCGATTGACTCATCAGGCAAACTTACGTTTTTCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11