Mutation ilvC725(del)::kan
- Name: ilvC725(del)::kan
- Type: Insertion
- Mutation of Gene: ilvC
- Approx. Map Location: 85.26
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AGAGTCGGGGTGAGGGCATCAGCGCGCACTTAACCCGCAACAGCAATACGATTCCGGGGATCCGTCGACC and CAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3747-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔilvC725::kan, Δ(rhaD-rhaB)568, hsdR514