Mutation intF725(del)::kan
- Name: intF725(del)::kan
- Type: Insertion
- Mutation of Gene: intF
- Approx. Map Location: 6.36
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATTTTGTAATATGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTATTCCGGGGATCCGTCGACC and CACCAAGTTATAGCAATAAACCCGTTGATTGAAATTAACAAAAGTTGATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0275-1 7 F-, Δ(araD-araB)567, ΔintF725::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514