Mutation intQ726(del)::kan
- Name: intQ726(del)::kan
- Type: Insertion
- Mutation of Gene: intQ
- Approx. Map Location: 35.55
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATTCCGGGGATCCGTCGACC and TGCGTGTTAACTATTTGATAAATATTAAATTAATTTTTCATTGCTTCGTTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1571-4 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔintQ726::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514