Mutation kefF759(del)::kan
- Name: kefF759(del)::kan
- Type: Insertion
- Mutation of Gene: kefF
- Approx. Map Location: 1.02
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCCGGGGATCCGTCGACC and GATAAATCAGCGCCTGAATCAGCGTATGGCTATCCATGATGGGCCTCCTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0045-1 7 F-, ΔkefF759::kan, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514