Mutation lysU756(del)::kan
- Name: lysU756(del)::kan
- Type: Insertion
- Mutation of Gene: lysU
- Approx. Map Location: 93.78
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCATTCCGGGGATCCGTCGACC and GTCTCTTTATCTACGCTAAATTGAAAGCTGGATTTAGAGGAACCAAAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4090-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔlysU756::kan, hsdR514