Mutation mcrA747(del)::kan
- Name: mcrA747(del)::kan
- Type: Insertion
- Mutation of Gene: mcrA
- Approx. Map Location: 26.07
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TCAATAAAAGTAGTATTGTCGTGAAAAATTGATTAAAGATTAATATTATGATTCCGGGGATCCGTCGACC and CGTCTTAAATGTGACTTTAATAAATAATTTTATTTCTGTAATCGGTTTATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1145-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔmcrA747::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514