Mutation metJ725(del)::kan
- Name: metJ725(del)::kan
- Type: Insertion
- Mutation of Gene: metJ
- Approx. Map Location: 88.93
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CTGTGTGGTCTGGTCTCAATTTATTGACGAAGAGGATTAAGTATCTCATGATTCCGGGGATCCGTCGACC and GATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3909-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔmetJ725::kan, hsdR514