Mutation nrdE756(del)::kan
- Name: nrdE756(del)::kan
- Type: Insertion
- Mutation of Gene: nrdE
- Approx. Map Location: 60.34
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GGTACGCAAAGCGATATCGAAAACGTTCGTAAAGGAGTAACCGAATTTTGATTCCGGGGATCCGTCGACC and CTGATACGTGAGAGTTTCATAGATATTCCTTAAAGTGCACAGGAGACGCATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2650-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔnrdE756::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514