Mutation potI758(del)::kan
- Name: potI758(del)::kan
- Type: Insertion
- Mutation of Gene: potI
- Approx. Map Location: 19.32
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGATGATTCCGGGGATCCGTCGACC and TGTAGCCAGGTTGGCAAATTTTAGTGTCTTCAGCCACGTCTTGCACGCTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0841-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpotI758::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514