Mutation ppdD726(del)::kan
- Name: ppdD726(del)::kan
- Type: Insertion
- Mutation of Gene: ppdD
- Approx. Map Location: 2.52
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGATTCCGGGGATCCGTCGACC and GATGGCGTTTGCCAAAGTAGCGCCAACCAAATCAAGGAGCGAAACAGATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0104-1 7 F-, Δ(araD-araB)567, ΔppdD726::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514