Mutation puuA733(del)::kan
- Name: puuA733(del)::kan
- Type: Insertion
- Mutation of Gene: puuA
- Approx. Map Location: 29.26
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCAATTCCGGGGATCCGTCGACC and TTTTACGCTTTGATAACGAGCGGAAAACAAACCAAAGGCGAAGAATCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5201-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpuuA733::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514