Mutation rnd-729(del)::kan
- Name: rnd-729(del)::kan
- Type: Insertion
- Mutation of Gene: rnd
- Approx. Map Location: 40.63
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAAATTCCGGGGATCCGTCGACC and CCGGTTAATCCGGCGTTTTTTTTGACGCCCACTAAAGAGAAAACAATTTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1793-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δrnd-729::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514