Mutation smpB776(del)::kan
- Name: smpB776(del)::kan
- Type: Insertion
- Mutation of Gene: smpB
- Approx. Map Location: 59.34
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGATTCCGGGGATCCGTCGACC and AGGAACTGGTCAATAATTGGAGTGCAGGTTTAACGGTGGGCGTTTTTCATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2601-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔsmpB776::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514