Mutation - spy-741(del)::kan

Mutation - spy-741(del)::kanMutation spy-741(del)::kan

Mutation spy-741(del)::kan

Name: spy-741(del)::kan
Type: Insertion
Mutation of Gene: spy
Approx. Map Location: 39.30
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were CGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATATTCCGGGGATCCGTCGACC and CTACTTAAGAAAGCCGTAATAAATAACTGAAAGGAAGGATATAGAATATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
1 Strains Carrying This Mutation
Name Mutations Genotype
JW1732-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, Δspy-741::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514

Property	Symbol
deletion	del
kanamycin resistant