Mutation thiD728(del)::kan
- Name: thiD728(del)::kan
- Type: Insertion
- Mutation of Gene: thiD
- Approx. Map Location: 47.03
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGATTCCGGGGATCCGTCGACC and CCACATTTCCTTGATGCGCTCTGGCAATTGACGCAGGAGGTGCAGGCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2090-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔthiD728::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514