Mutation tolB789(del)::kan
- Name: tolB789(del)::kan
- Type: Insertion
- Mutation of Gene: tolB
- Approx. Map Location: 16.75
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGATTCCGGGGATCCGTCGACC and TGATTCCTTTACTATTCAATTAATTATTATCACAGATACGGCGACCAGGCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5100-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔtolB789::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514