Mutation ung-748(del)::kan
- Name: ung-748(del)::kan
- Type: Insertion
- Mutation of Gene: ung
- Approx. Map Location: 58.52
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AAAGAAGCAGTTAAGCTAGGCGGATTGAAGATTCGCAGGAGAGCGAGATGATTCCGGGGATCCGTCGACC and ACTCTGCCATCCGGCATTTCCCCGCAAATTTACTCACTCTCTGCCGGTAATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2564-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δung-748::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514