Mutation wcaA757(del)::kan
- Name: wcaA757(del)::kan
- Type: Insertion
- Mutation of Gene: wcaA
- Approx. Map Location: 45.92
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCATTCCGGGGATCCGTCGACC and CGCCGCATCCGGCAAGCAAACCAGCTCATAAGCCGGGAGAACAACCTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2044-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔwcaA757::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514