Mutation ycbB742(del)::kan
- Name: ycbB742(del)::kan
- Type: Insertion
- Mutation of Gene: ycbB
- Approx. Map Location: 21.13
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AATAACAGCCTGGCTATTCAGAGTATGATAAAAACAGGGGGCAAGGGATGATTCCGGGGATCCGTCGACC and CGACAACTGATTTCCCCGAACTACTTCATTTACCTGATTAATTGTTCCGCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0908-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔycbB742::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514