Mutation yccE745(del)::kan
- Name: yccE745(del)::kan
- Type: Insertion
- Mutation of Gene: yccE
- Approx. Map Location: 22.92
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AGTTTAACTAGGGCGTCATTATTTATTAAATTTTATAGACGCTATATATGATTCCGGGGATCCGTCGACC and ACAATGGTTTTTAGCATATGCCTTTGCCATTAAATTGCATAACGATTTAATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0986-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyccE745::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514