Mutation yfiR766(del)::kan
- Name: yfiR766(del)::kan
- Type: Insertion
- Mutation of Gene: yfiR
- Approx. Map Location: 59.05
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGATTCCGGGGATCCGTCGACC and ACGTGGGGCGCTTATTAAGAGAATTATCGTTATCCATCATTTTTCTTCCGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2584-5 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyfiR766::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514