Mutation ykfJ753(del)::kan
- Name: ykfJ753(del)::kan
- Type: Insertion
- Mutation of Gene: ykfJ
- Approx. Map Location: 5.46
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AACGAACGCAGTCGCAGTACATGCAACTTGAAGTATGACGAGTATAAATGATTCCGGGGATCCGTCGACC and CGAGAGAAACCAGCGCCGAACGCAGTGTGTCAGAGTAGCGGCCCGTTTCTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0225-1 7 F-, Δ(araD-araB)567, ΔykfJ753::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514