Mutation - clsC788(del)::kan

Mutation - clsC788(del)::kanMutation clsC788(del)::kan

Mutation clsC788(del)::kan

Name: clsC788(del)::kan
Type: Insertion
Mutation of Gene: clsC
Approx. Map Location: 23.83
Synonyms
Designation Where Used
ymdC788(del)::kan
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were CGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGATTCCGGGGATCCGTCGACC and CGATAAAGCACCAGCCCGTTAAGCCACATTTACAATAACCATTCCACGGGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11

Designation	Where Used
ymdC788(del)::kan

Property	Symbol
deletion	del
kanamycin resistant

2 Strains Carrying This Mutation

Name	Mutations	Genotype
BKT12	6	F-, λ^-, ΔclsB861, ΔclsC788::kan, ΔclsA856, IN(rrnD-rrnE)1, rph-1
JW5150-1	7	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔclsC788::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514