Mutation gfcA731(del)::kan
- Name: gfcA731(del)::kan
- Type: Insertion
- Mutation of Gene: gfcA
- Approx. Map Location: 22.60
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AACTTTAATTTCGACGCGTTAAAGCAGTATTAGCGTGTAGTGGACGTGGTATTCCGGGGATCCGTCGACC and ATACACTAAATCAGTAAGTTGGCAGCATCACCCGTATTGTCCATGCGATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5133-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔgfcA731::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514