Mutation pgl-739(del)::kan
- Name: pgl-739(del)::kan
- Type: Insertion
- Mutation of Gene: pgl
- Approx. Map Location: 17.20
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ACTAATTTTAGCTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGATTCCGGGGATCCGTCGACC and AGGAGAGACTGCGCCGGGTAAATCAGCGGTTAGTGTGCGTTAACCACCACTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0750-3 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δpgl-739::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514