Mutation tfaD770(del)::kan
- Name: tfaD770(del)::kan
- Type: Insertion
- Mutation of Gene: tfaD
- Approx. Map Location: 12.52
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGATTCCGGGGATCCGTCGACC and TAAGACGTGCTGCGGCATAATCCCAATGATTACTCCCTGACAGGGTTCGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5815-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔtfaD770::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514