Mutation puuE738(del)::kan
- Name: puuE738(del)::kan
- Type: Insertion
- Mutation of Gene: puuE
- Approx. Map Location: 29.39
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGATTCCGGGGATCCGTCGACC and AGCGCATCAGACATTATTGCGTTGGGCTATTAATCGCTCAGCGCATCCTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1295-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpuuE738::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514