Mutation - thyA762(del)::kan

Mutation - thyA762(del)::kanMutation thyA762(del)::kan

Mutation thyA762(del)::kan

Name: thyA762(del)::kan
Type: Insertion
Mutation of Gene: thyA
Approx. Map Location: 63.86
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- The primers used to make this knockout were GGCGTCGGCTCTGGCAGGATGTTTCGTAATTAGATAGCCACCGGCGCTTTATTCCGGGGATCCGTCGACC and TCTGGGCATATCGTCGCAGCCCACAGCAACACGTTTCCTGAGGAACCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11

Property	Symbol
deletion	del
kanamycin resistant