Mutation gsiD732(del)::kan
- Name: gsiD732(del)::kan
- Type: Insertion
- Mutation of Gene: gsiD
- Approx. Map Location: 18.78
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GATGTGCTTTACGCCGCCATTAACCCGGCTATCAGGTACAAGTAAGGATGATTCCGGGGATCCGTCGACC and TTACCCTTTCAAGCAATATTCAAACGTAACTATCCTTTAATTTTCGGATCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0816-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔgsiD732::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514