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Mutation cfa-5::kan
- Name: cfa-5::kan
- Type: Tn
- Mutation of Gene: cfa
- Approx. Map Location: 37.49
- Properties
Property Symbol kanamycin resistant - Comments
- Plasmid pAYW19, Wang et al., 1992, carrying cfa was cut with KpnI (at cfa codon 17 of 382),ligated to 2kbp containing kanR gene of pHP45omega-Km(Fellay et al.1987);used to transform JC7623
- References
- Wang, A.Y., D.W. Grogan, J.E. Cronan 1985. Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site. Biochemistry 31:11020-11028
- Fellay, R., J. Frey, H.M. Krisch 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52:147-154
- Chang, Y.Y., J.E. Cronan 1999. Membrane cyclopropane fatty acid content is a major factor in acid resistance of Escherichia coli. Mol.Microbiol. 33:249-259
- 1 Strains Carrying This Mutation
Name Mutations Genotype YYC1272 6 F-, Δ(argF-lac)169, λ-, cfa-5::kan, IN(rrnD-rrnE)1, rph-1, tnaA5?