Mutation afuB'-780(del)::kan
- Name: afuB'-780(del)::kan
- Type: Insertion
- Mutation of Gene: afuB'
- Approx. Map Location: 5.99
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTATTCCGGGGATCCGTCGACC and TGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0255-4 7 F-, Δ(araD-araB)567, ΔafuB'-780::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514