Mutation agaD754(del)::kan
- Name: agaD754(del)::kan
- Type: Insertion
- Mutation of Gene: agaD
- Approx. Map Location: 70.77
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGATTCCGGGGATCCGTCGACC and GCACCACCAGACGCAGTGCCTCGTTCCATTTACAAAATGCCGAATGCGGATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3109-3 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔagaD754::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514