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Site thyA
- Name: thyA
- Type: Gene
- Synonyms
b2827 MG1655 locus tag - Mnemonic: Thymine
- Left End Point: 63.86
- Right End Point: 63.87
- Products:
thymidylate synthetase - Direction: <
- Properties:
Property Comment auxotrophy-pur/pyr NULL aminopterin resistance NULL - Priority: 1
- External Database Links:
Host Site Page Links NCBI/GenBank J01710 EcoGene.org EG11002 EcoliWiki thyA - Comment:
- Selectable phenotype. Loss: aminopterin resistance; trimethoprim resistance; may be nonsense suppressor. Unless thy-supplem.is used, even with rich media, for thyA muts ->deoB or C, ->low-thy gr.
- References:
- Bertino, J.B., K.A. Stacey 1966. A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli. Biochem.J. 101:32-33
- Caster, J.H. 1967. Selection of thymine-requiring strains from Escherichia coli on solid medium. J.Bacteriol. 94:1804
- Rubin, E.M., G. Wilson, F.E. Young 1980. Expression of thymidylate synthetase activity in Bacillus subtilis upon integration of a cloned gene from E. coli. Gene 10:227-235
- Hickson, I.D., K.E. Atkinson, P.T. Emmerson 1982. Molecular cloning and amplification of the gene for thymidylate synthetase. Gene 18:257-260
- Sasaki, M., T. Fujiyoshi, K. Shimada, Y. Takagi 1982. Fine structure of the recB and recC gene region of Escherichia coli. Biochem.Biophys.Res.Commun. 109:414-422
- Belfort, M., G. Maley, J. Pederson-Lane, F. Maley 1983. Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product. Proc.Natl.Acad.Sci.USA 80:4914-4918
- Herrington, M.B., A. Kohli, P.H. Lapchak 1984. Suppression by thymidine-requiring mutants of Escherichia coli K-12. J.Bacteriol. 157:126-129
- Dykstra, C.C., D. Prasher, S.R. Kushner 1984. Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12. J.Bacteriol. 157:21-27
- Nakano, H., S. Goto, T. Nakayashiki, H. Inokuchi 2001. Temperature-sensitive mutations in various genes of Escherichia coli K12 can be suppressed by the ssrA gene for 10Sa RNA (tmRNA). Molecular Genetics & Genomics: MGG 265:615-621