E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection
• The Database
• Privacy Policy
• Acknowledgment of Support

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Contact Information
• Charges
• Reviving Strains
• Strains Not Found?

Other CGSC Information

• FAQ on Procedures
• Current Working Map
• 1998 MMBR Map
• 1998 MMBR Gene List
• Map Diagrams (PDFs)

Other Links

• E. coli Links
• Other Stock Collections
• Other Bio Links
• Yale Web

Funded by a grant from the National Science Foundation

Site pLC32-25

• Name: pLC32-25
• Type: Plasmid
  SubSites:

  guaA

  guaB

  
  
  Plasmid Sites:

  araC14

  galK2(Oc)

  galT22

  glnX44(AS)

  lacY1

  LAM-

  leuB6(Am)

  mtl-1

  recA56

  thiE1

  thr-1

  trpE63(del)

  xylA5

  
  
• Priority: 0
• Comment:
• References:
  • Gots, J.S. 0. pers. comm. to J. Carbon. Personal Commun. 00:0-0
  
  
  • Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  pLC32-25	13	F+, thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, pLC32-25