Site putA
- Name: putA
- Type: Gene
- Synonyms
b1014 MG1655 locus tag poaA - Mnemonic: proline utilization
- Left End Point: 23.15
- Right End Point: 23.24
- Products:
proline oxidase multifctnl. proline dehydrogenase, pyrroline-5-carboxylate deHase, put repressor - Direction: <
- Properties:
Property Comment autogenously regulated NULL encodes membrane protein as memb.prot.,oxidizes Pro to Glutamate encodes NA-binding protein in cytopl., represses put transcr. - Priority: 1
- 4 Alleles of This Gene
putA11 putA1::Tn5 putA758(del)::kan putA791-YFP(::cat) - External Database Links:
Host Site Page Links EcoGene.org EG10801 EcoliWiki putA - Comment:
- Sel. phen. Loss: putA-, P-, azetidine carboxylate res. (Still dehydroproline sens); putA+, P-, azetidine carboxylate, dehy- droproline res.; indicator plate putP-, dehydroproline res.
- References:
- Tristram, H., S. Neale 1968. The activity and specificity of the proline permease in wild-type and analogue-resistant strains of Escherichia coli. J.Gen.Microbiol. 50:121-137
- Condamine, H. 1971. Sur la regulation de la production de proline chez E. coli K-12. Ann.Inst.Pasteur 120:126-143
- Wolf-Watz, H., M. Masters 1979. Deoxyribonucleic acid and outer membrane: strains diploid for the oriC region show elevated levels of deoxyribonucleaic acid-binding protein and evidence for specific binding of the oriC region to outer membrane. J.Bact
- Wood, J.M., D. Zadworny 1980. Amplification of the put genes and identification of the put gene products in Escherichia coli K12. Can.J.Biochem. 58:787-796
- Stalmach, M.E., S.R. Grothe, J.M. Wood 1983. Two proline porters in Escherichia coli K-12. J.Bacteriol. 156:481-486
- Rancourt, D.E., J.T. Stephenson, G.A. Vickell, J.M. Wood 1984. Proline excretion by Escherichia coli K12. Biotechnol.Bioeng. 26:74-80
- Mogi, T., H. Yamamoto, T. Nakao, I. Yamato, Y. Anraku 1986. Genetic and physical characterization of putP, the proline carrier gene of Escherichia coli K12. Mol.Gen.Genet. 202:35-41
- Nakao, T., I. Yamato, Y. Anraku 1987. Nucleotide sequence of putC, the regulatory region for the put regulon of Escherichia coli K12. Mol.Gen.Genet. 210:364-368
- Deutch, C.E. 1992. Oxidation of L-thiazolidine-4-carboxylate by L-proline dehydrogenase in Escherichia coli. J.Gen.Microbiol. 138:1593
- Maloy, S.R., V.J. Stewart 1993. Autogenous regulation of gene expression. J.Bacteriol. 175:307-316