Site ompF
- Name: ompF
- Type: Gene
- Synonyms
b0929 MG1655 locus tag cmlB coa cry tolF - Mnemonic: outer membrane protein
- Left End Point: 21.24
- Right End Point: 21.26
- Products:
outer membrane protein 1a (Ia;b;F), structural gene porin, non-specific pore protein of OM - Direction: <
- Priority: 1
- 7 Alleles of This Gene
ompF254 ompF480(del) ompF625 ompF626 ompF627(T2R) ompF746(del)::kan ompF846(del)::FRT - External Database Links:
Host Site Page Links NCBI/GenBank J01655 EcoGene.org EG10671 EcoliWiki ompF - Comment:
- Selectable phenotype. Loss: phage Me1, phage TuIa resistance; chloramphenicol, tetracycline resistance; phage PH51, phage PH105 resistance; resistance to 5-fluorouracil + a nucleotide.
- References:
- Reeve, E.C.R. 1968. Genetic analysis of some mutations causing resistance to tetracycline in Escherichia coli K12. Genet.Res. 11:303-309
- Beacham, I.R., R. Kahana, L. Levy, E. Yagil 1973. Mutants of Escherichia coli K-12 "cryptic" or deficient in 5'-nucleotidase (uridine diphosphate-sugar hydrolase) and 3'-nucleotidase (cyclic phosphodiesterase) activity. J.Bacteriol. 116:957-964
- Verhof, C., B. Lugtenberg, R. van Boxtel, P. de Graaff, H.M. Verheij 1979. Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K12. Mol.Gen.Genet. 169:137-146
- Sato, T., T. Yura 1981. Regulatory mutations conferring constitutive synthesis of major outer membrane proteins (OmpC and OmpF) in Escherichia coli. J.Bacteriol. 145:88-96
- Mutoh, N., T. Nagasawa, S. Mizushima 1981. Specialized transducing bacteriophage lambda carrying the structural gene for a major outer membrane matrix protein of Escherichia coli K-12. J.Bacteriol. 145:1085-1090
- Mutoh, N., K. Inokuchi, S. Mizushima 1982. Amino acid sequence of the signal peptides of OmpF, a major outer membrane protein of Escherichia coli. FEBS Lett. 137:171-174
- Inokuchi, K., N. Mutoh, S. Matsuyama, H. Mizushima 1982. Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res. 10:6957-6968
- Tommassen, J., P. van der Ley, A. van der Ende, H. Bergmans, B. Lugtenberg 1982. Cloning of ompF, the structural gene for an outer membrane pore protein of Escherichia coli K12: physical localization and homology with the phoE gene. Mol.Gen.Genet. 185
- Inokuchi, K., H. Furukawa, H. Nakamura, H. Mizushima 1984. Characterization by deletion mutagenesis in vitro of the promoter region of ompF, a positively regulated gene of Escherichia coli. J.Mol.Biol. 178:653-668
- Dairi, T., K. Inokuchi, T. Mizuno, H. Mizushima 1985. Positive control of transcription initiation in Escherichia coli. A base substitution at the Pribnow box renders ompF expression independent of a positive regulator. J.Mol.Biol. 184:1-6
- Taylor, R.K., S. Garrett, E.J. Sodergren, T.J. Silhavy 1985. Mutations that define the promoter of ompF, a gene specifying a major outer membrane porin protein. J.Bacteriol. 162:1054-1060
- Malcolm, B.A., J.F. Kirsch 1985. Site-directed mutagenesis of aspartate aminotransferase from E. coli. Biochem.Biophys.Res.Commun. 132:915-921
- Tsui, P., V. Helu, M. Freundlich 1988. Altered osmoregulation of ompF in integration host factor mutants of Escherichia coli. J.Bacteriol. 170:4950-4953
- Rosner, J.L., T.J. Chai, J. Foulds 1991. Regulation of ompF porin expression by salicylate in Escherichia coli. J.Bacteriol. 173:5631-5638
- Misra, R. 1993. A novel ompC mutation of Escherichia coli K12 that reduces OmpC and OmpF levels in the outer membrane. Mol.Microbiol. 10:1029-1035
- Pugsley, A.P. 1993. A mutation in the dsbA gene coding for periplasmic disulfide oxidoreductase reduces transcription of the Escherichia coli ompF gene. Mol.Gen.Genet. 237:407-411
- Huang, K.-J., J.L. Schieberl, M.M. Igo 1994. A distant upstream site involved in the negative regulation of the Escherichia coli ompF gene. J.Bacteriol. 176:1309-1315
- Harlocker, S.L., L. Bergstrom, M. Inouye 1995. Tandem binding of six OmpR proteins to the ompF upstream regulatory sequence of Escherichia coli. J.Biol.Chem. 270:26849-26856
- Forst, S., I. Kalve, W. Durski 1995. Molecular analysis of OmpR binding sequences involved in the regulation of ompF in Escherichia coli. FEMS Microbiol.Lett. 131:147-0
- Huang, K.-J., M.M. Igo 1996. Identification of the bases in the ompF regulatory region, which interact with the transcription factor OmpR. J.Mol.Biol. 262:615-628
- Nurminen, M., L. Hirvas, M. Vaara 1997. The outer membrane of lipid A-deficient Escherichia coli mutant LH530 has reduced levels of OmpF and leaks periplasmic enzymes. Microbiology 143:1533-1537
- Bergstrom, L.C., L. Qin, S.L. Harlocker, L.A. Egger, M. Inouye 1998. Hierarchical and co-operative binding of OmpR to a fusion construct containing the ompC and ompF upstream regulatory sequences of Escherichia coli. Genes Cells 3:777-788
- Liu, X., T. Ferenci 2001. An analysis multifactorial influences on the transcriptional control ompF and ompC porin expression under nutrient limitation. Microbiology 147:2981-2989