Site ilvG
- Name: ilvG
- Type: Gene
- Synonyms
b4488 MG1655 locus tag - Mnemonic: Isoleucine-valine (requirement)
- Left End Point: 85.10
- Right End Point: 85.14
- Products:
AHAS II, acetohydroxy acid synthase, valine-insensitive acetolactate synthase II, valine-insensitive, ALS II(inactive in K-12, frameshift) - Direction: >
- Properties:
Property Comment `silent' Frameshift mutation present in K-12 wildtype - Priority: 1
- 8 Alleles of This Gene
ilvG468(Act) ilvG603(Act) ilvG605(Am) ilvG789(del)::kan ilvG790(del)::kan ilvG865(Act) ilvG866(Act) ilvG867(Act) - External Database Links:
Host Site Page Links NCBI/GenBank V00289 EcoGene.org EG10498 EcoliWiki ilvG - Comment:
- Selectable phenotype. Gain of activity: valine resistance. Inactive in K-12 due to frameshift mutation.
- References:
- Glover, S.W. 1962. Valine-resistant mutants of Escherichia coli K-12. Genet.Res. 3:448-460
- Lawther, R.P., B. Nichols, G. Zurawski, G.W. Hatfield 1979. The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli. Nucleic Acids Res. 7:2289-2301
- Berg, C.M., K.J. Shaw, D.E. Berg 1980. The ivlG gene is expressed in Escherichia coli K-12. Gene 12:165-170
- Subrahmanyam, C.S., G.M. McCorkle, H.E. Umbarger 1980. Physical location of the ilvO determinant in Escherichia coli K-12 deoxyribonucleic acid. J.Bacteriol. 142:547-555
- Nargang, F.E., C.S. Subrahmanyam, H.E. Umbarger 1980. Nucleotide sequence of ivlGEDA operon attenuator region of Escherichia coli. Proc.Natl.Acad.Sci.USA 77:1823-1827
- Lawther, R.P., G.W. Hatfield 1980. Multivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12. Proc.Natl.Acad.Sci.USA 77:1862-1866
- Gayda, R.C., T.D. Leathers, J.D. Noti, F.J. Smith, C.S. Subrahmanyam, H.E. Umbarger 1980. Location of the multivalent control site for the ilvEDA operon of Escherichia coli. J.Bacteriol. 142:556-567
- Subrahmanyam, C.S., J.D. Noti, H.E. Umbarger 1980. Regulation of ilvEDA expression occurs upstream of ilvG in Escherichia coli: additional evidence for an ilvGEDA operon. J.Bacteriol. 144:279-290
- Lawther, R.P., D.H. Calhoun, C.W. Adams, C.A. Hauser, J.E. Gray, G.W. Hatfield 1981. Molecular basis of valine resistance in Escherichia coli K-12. Proc.Natl.Acad.Sci.USA 78:922-925
- Uzan, M., R. Favre, E. Gallay, L. Caro 1981. Genetical and structural analysis of a group of lambda ilv and lambda rho transducing phages. Mol.Gen.Genet. 182:462-470
- Lawther, R.P., D.H. Calhoun, J.E. Gray, C.W. Adams, C.A. Hauser, G.W. Hatfield 1982. DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12. J.Bacteriol. 149:294-298
- Gray, J.E., D.C. Bennett, H.E. Umbarger, D.H. Calhoun 1982. Physical and genetic localization of ilv regulatory sites in LAM ilv bacteriophages. J.Bacteriol. 149:1071-1081
- Driver, R.P., R.P. Lawther 1985. Physical analysis of deletion mutations in the ilvGEDA operon of Escherichia coli K-12. J.Bacteriol. 162:598-606
- Lu, M.-F., H.E. Umbarger 1987. Effects of deletion and insertion mutations in the ilvM gene of Escherichia coli. J.Bacteriol. 169:600-604
- Lawther, R.P., R.C. Wek, J.M. Lopes, R. Pereira, B.E. Taillon, G.W. Hatfield 1987. The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res. 15:2137-2155
- Cox, J.L., B.J. Cox, V. Fidanza, D.H. Calhoun 1987. The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12. Gene 56:185-198
- Aldea, M., V.F. Maples, S.R. Kushner 1988. Generation of a detailed physical and genetic map of the ilv-metE-udp region of the Escherichia coli chromosome. J.Mol.Biol. 200:427-438
- Daniels, D.L., G. Plunkett, V.D. Burland, F.R. Blattner 1992. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science 257:771-778
- Huang, F., G. Coppola, D.H. Calhoun 1992. Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12. J.Bacteriol. 174:4871-4877
- Burns, D.M., M.J. Burger, I.R. Beacham 1995. Silent genes in bacteria: the previously designated 'cryptic' ilvHI locus of 'Salmonella typhimurium LT2' is active in natural isolates. FEMS Microbiol.Lett. 131:167-172
- Parekh, B.S., G.W. Hatfield 1997. Growth rate-related regulation of the ilvGMEDA operon of Escherichia coli K-12 is a consequence of the polar frameshift mutation in the ilvG gene of this strain. J.Bacteriol. 179:2086-2088