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Site pLC32-12D
- Name: pLC32-12D
- Type: Plasmid
- SubSites:
trpA trpB trpC trpD trpE topA cysB - Plasmid Sites:
araC14 galK2(Oc) galT22 glnX44(AS) lacY1 LAM- leuB6(Am) mtl-1 recA56 thiE1 thr-1 trpE63(del) xylA5 - Priority: 0
- Comment:
- Mixed clone. See also pLC32-12A, pLC32-12B, and pLC32-12C. Hybridizes with Kohara clones 252-254, 119, 146, and 450. Similar hybridization pattern to pLC5-23, pLC4-6, pLC32-27, and pLC12-34.
- References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
- 1 Strains Carrying This Plasmid
Name Mutations Genotype pLC32-12D 13 F+, thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, pLC32-12D