Site dnaQ
- Name: dnaQ
- Type: Gene
- Synonyms
b0215 MG1655 locus tag mutD - Mnemonic: DNA
- Left End Point: 5.09
- Right End Point: 5.10
- Products:
DNA polymerase III epsilon subunit DNA polymerase III E subunit of holoenzyme, mutator activity - Direction: <
- Properties:
Property Comment SOS-related ? modest,slow induction under some DNA damage conditions, requiring recA and lexA for transcriptional induction, but promoter has no LexA box - Priority: 1
- 3 Alleles of This Gene
dnaQ49(ts) dnaQ744(del)::kan dnaQ905 - External Database Links:
Host Site Page Links NCBI/GenBank K00985 EcoGene.org EG10243 EcoliWiki dnaQ - Comment:
- Selectable phenotype. Streptomycin resistance; azaserine resistance; chemostat growth. 3' to 5' exonucleolytic proofreading
- References:
- Siegel, E., V. Bryson 1967. Mutator gene of Escherichia coli. J.Bacteriol. 94:38-47
- Helling, R.B. 1968. Selection of a mutant of Escherichia coli which has high mutation rates. J.Bacteriol. 96:975-980
- Cox, E.C., T. Gibson 1974. Selection for high mutation rates in chemostats. Genetics 77:169-184
- Horiuchi, T., H. Maki, M. Maruyana, M. Sekiguchi 1981. Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes. Proc.Natl.Acad.Sci.USA 78:3770-3774
- Scheurmann, R., S. Tam, P.M.J. Burgers, C. Lu, H. Echols 1983. Identification of the E-subunit of Escherichia coli DNA polymerase III holoenzyme as the dnaQ gene product: a fidelity subunit for DNA replication. Proc.Natl.Acad.Sci.USA 80:7085-7089
- Maruyama, M., T. Horiuchi, H. Maki, M. Sekiguchi 1983. A dominant (mutD5) and a recessive (dnaQ49) mutator of Escherichia coli. J.Mol.Biol. 167:757-771
- Maki, H., T. Horiuchi, M. Sekiguchi 1983. Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions. Proc.Natl.Acad.Sci.USA 80:7137-7141
- Echols, H., C. Lu, P.M.J. Burgers 1983. Mutator strains of Escherichia coli, mutD and dnaQ, with defective exonucleolytic editing by DNA polymerase III holoenzyme. Proc.Natl.Acad.Sci.USA 80:2189-2192
- Maki, H., T. Horiuchi, M. Sekiguchi 1983. Isolation of conditional lethal mutator mutants of Escherichia coli by localized mutagenesis. J.Bacteriol. 153:1361-1367
- DiFrancesco, R., S.K. Bhatnagar, A. Brown, M.J. Bessman 1984. The interaction of DNA polymerase III and the product of the Escherichia coli mutator gene, mutD. J.Biol.Chem. 259:5567-5573
- Nomura, T., H. Aiba, A. Ishihama 1985. Transcriptional organization of the convergent overlapping dnaQ-rnh genes of Escherichia coli. J.Biol.Chem. 260:7122-7125
- Takano, K., Y. Nakabeppu, H. Maki, T. Horiuchi, M. Sekiguchi 1986. Structure and function of dnaQ and mutD mutators of Escherichia coli. Mol.Gen.Genet. 205:9-13
- Cox, E.C., D.L. Horner 1986. DNA sequence and coding properties of mutD (dnaQ) a dominant Escherichia coli mutator gene. J.Mol.Biol. 190:113-117
- Clementz, T. 1992. The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli. J.Bacteriol. 174:7750-7756
- Oller, A.R., I.J. Fijalkowska, R.M. Schaaper 1993. The Escherichia coli galK2 papillation assay: its specificity and application to seven newly isolated mutator strains. Mutat.Res. 292:175-185
- Kelman, Z., M.A. O'Donnell 1995. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu.Rev.Biochem. 64:171-200
- Fijalkowska, I.J., R.M. Schaaper 1995. Effects of Escherichia coli dnaE antimutator alleles in a proofreading-deficient mutD5 strain. J.Bacteriol. 177:5979-5986
- Fijalkowska, I.J., R.L. Dunn, R.M. Schaaper 1997. Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity. J.Bacteriol. 179:7435-7445
- Quinones, A., S. Neumann 1997. The ssb-113 allele suppresses the dnaQ49 mutator and alters DNA supercoiling in Escherichia coli. Mol.Microbiol. 25:237-246
- Jonczyk, P., A. Nowicka, I.J. Fijalkowska, R.M. Schaaper, Z. Ciesla 1998. In vivo protein interactions within the Escherichia coli DNA polymerase III core. J.Bacteriol. 180:1563-1566