Site dnaK
- Name: dnaK
- Type: Gene
- Synonyms
b0014 MG1655 locus tag groPAB groPC groPF grpF seg Ezaki et al. - Mnemonic: DNA
- Left End Point: 0.26
- Right End Point: 0.30
- Products:
Hsp70, chaperone - Direction: >
- Properties:
Property Comment stress response heat shock, heat shock regulon NULL encodes chaperone molecule NULL autogenously regulated autoregulation of heat shock response - Priority: 1
- 3 Alleles of This Gene
dnaK734(del)::kan dnaK756(ts) dnaK795-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank K01298 NCBI/GenBank M10420 NCBI/GenBank M12544 EcoGene.org EG10241 EcoliWiki dnaK - Comment:
- DNA biosynthesis. ATP-regulated binding and release of polypeptide substrates; moleclar chaperone
- References:
- Bardwell, J.C., E.A. Craig 1984. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous. Proc.Natl.Acad.Sci.USA 81:848-852
- Paek, KH, GC Walker 1987. Escherichia coli dnaK null mutants are inviable at high temperature. J. Bacteriol. 169(1):283-90.
- Sakakibara, Y. 1988. The dnaK gene of Escherichia coli functions in initiation of chromosome replication. J.Bacteriol. 170:972-979
- Ezaki, B., T. Ogura, H. Mori, H. Niki, S. Hiraga 1989. Involvement of DnaK protein in mini-F plasmid replication: temperature-sensitive seg mutations are located in the dnaK gene. Mol.Gen.Genet. 218:183-189
- Ohki, R., R. Morita, T. Kawamata, H. Uchida, M. Ohki 1989. A complete deletion mutant of the Escherichia coli dnaKdnaJ operon. Biochim.Biophys.Acta 1009:94-98
- Straus, D., W. Walter, C.A. Gross 1990. DnaK, DnaJ, and GrpE heat shock proteins negatively regulate heat shock gene expression by controlling the synthesis and stability of sigma 32. Genes Dev. 4:2202-2209
- Ezaki, B., H. Mori, T. Ogura, S. Hiraga 1990. Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli. Mol.Gen.Genet. 223:361-368
- Shi, W., Y. Zhou, J. Wild, J. Adler, C.A. Gross 1992. DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli. J.Bacteriol. 174:6256-6263
- Kanemori, M., H. Mori, T. Yura 1994. Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma-32 in Escherichia coli. J.Bacteriol. 176:5648-5653
- McCarty, J.S., G.C. Walker 1994. DnaK mutants defective in ATPase activity are defective in negative regulation of the heat shock response: expression of mutant DnaK proteins results in filamentation. J.Bacteriol. 176:764-780
- el Yaagoubi, A., M. Kohiyama, G. Richarme 1996. Defect in export and synthesis of the periplasmic galactose receptor MglB in dnaK mutants of Escherichia coli, and decreased stability of the mglB mRNA. Microbiology 142:2595-2602
- Miczak, A., V.R. Kaberdin, C.L. Wei, S. Lin-Chao 1996. Proteins associated with RNase E in a multicomponent ribonucleolytic complex. Proc.Natl.Acad.Sci.USA 93:3865-3869
- Neuhofen, S., H. Theyssen, J. Reinstein, W.E. Trommer, P.D. Vogel 1996. Nucleotide binding to the heat-shock protein DnaK as studied by ESR spectroscopy. Eur.J.Biochem. 240:78-82
- Burkholder, W.F., X. Zhao, X. Zhu, W.A. Hendrickson, A. Gragerov, M.E. Gottesman 1996. Mutations in the C-terminal fragment of DnaK affecting peptide binding. Proc.Natl.Acad.Sci.USA 93:10632-10637
- Muffler, A., M. Barth, C. Marschall, R. Hengge-Aronis 1997. Heat shock regulation of sigmaS turnover: a role for DnaK and relationship between stress responses mediated by sigmaS and sigma32 in Escherichia coli. J.Bacteriol. 179:445-452
- Pierpaoli, E.V., E. Sandmeier, H.J. Schonfeld, P. Christen 1998. Control of the DnaK chaperone cycle by substoichiometric concentrations of the co-chaperones DnaJ and GrpE. J.Biol.Chem. 273:6643-6649
- Rockabrand, D., K. Livers, T. Austin, R. Kaiser, D. Jensen, R. Burgess, P. Blum 1998. Roles of DnaK and RpoS in starvation-induced thermotolerance of Escherichia coli. J.Bacteriol. 180:846-854