E. coli Genetic Resources at Yale
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Strain JW0514-2

• CGSC#: 8657
• Strain Designation

  Designation	Source	Preference
  JW0514-2	Keio Collection	1
  JW0514	original Keio designation	2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  DE(araD-araB)567	1.40	1	Δ(araD-araB)567
  lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
  ppiB737(del)::kan	11.92	1	ΔppiB737::kan
  LAM-	17.40	1	λ-
  rph-1	82.20	1	rph-1
  DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
  hsdR514	98.74	1	hsdR514

  
  
• Comments:
  • From the Keio Collection of single-gene knockouts constructed through a collaboration of the Inst. of Adv. Biosci. at Keio Univ., the Nara Inst. of Sci. and Technol., Japan and Purdue Univ., USA
• References:
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
  
  

  Printable Strain Report